cellulase production by trichoderma reesei using sugar beet pulp

cellulase production by the fungus trichoderma reesei was studied using sugar beet pulp (sbp) as a substrate. the subculture medium was a salt solution consisting of kh2po4, cacl2, etc. fungal cells were sub-cultured in an orbital shaker (180 rpm) at 30°c for 1-2 generations (two days for each generation) and were then used as an inoculum. exponential cells were inoculated into a medium containing sbp. data showed that cellulose in sbp has a potential to be used as a substrate to produce cellulase. the effect of cellulose concentration on cellulase production was investigated. using the medium composition (the mixture of chemical salts and sbp substrate) and a fermentation time of 4-6 days, a maximum cellulase activity of 0.46 iu/ml of filter paper activity was obtained.